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medium  (MedChemExpress)


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    Structured Review

    MedChemExpress medium
    Medium, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 94/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/tpc2+a1+p/TPC2-A1-P/pm42584788-39-6-26
    Average 94 stars, based on 4 article reviews
    medium - by Bioz Stars, 2026-09
    94/100 stars

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    Related Articles

    Incubation:

    Article Title: Segregated cation flux by TPC2 biases Ca 2+ signaling through lysosomes
    Article Snippet: For some experiments, TPC2-A1-N and TPC2-A1-P were purchased from MedChem Express.

    Imaging:

    Article Title: Segregated cation flux by TPC2 biases Ca 2+ signaling through lysosomes
    Article Snippet: For some experiments, TPC2-A1-N and TPC2-A1-P were purchased from MedChem Express.

    Fluorescence:

    Article Title: Segregated cation flux by TPC2 biases Ca 2+ signaling through lysosomes
    Article Snippet: For some experiments, TPC2-A1-N and TPC2-A1-P were purchased from MedChem Express.

    Comparison:

    Article Title: Segregated cation flux by TPC2 biases Ca 2+ signaling through lysosomes
    Article Snippet: For some experiments, TPC2-A1-N and TPC2-A1-P were purchased from MedChem Express.

    Western Blot:

    Article Title: Segregated cation flux by TPC2 biases Ca 2+ signaling through lysosomes
    Article Snippet: For some experiments, TPC2-A1-N and TPC2-A1-P were purchased from MedChem Express.

    Construct:

    Article Title: Segregated cation flux by TPC2 biases Ca 2+ signaling through lysosomes
    Article Snippet: For some experiments, TPC2-A1-N and TPC2-A1-P were purchased from MedChem Express.

    Knock-Out:

    Article Title: Segregated cation flux by TPC2 biases Ca 2+ signaling through lysosomes
    Article Snippet: For some experiments, TPC2-A1-N and TPC2-A1-P were purchased from MedChem Express.

    Expressing:

    Article Title: Segregated cation flux by TPC2 biases Ca 2+ signaling through lysosomes
    Article Snippet: For some experiments, TPC2-A1-N and TPC2-A1-P were purchased from MedChem Express.

    Positive Control:

    Article Title: Segregated cation flux by TPC2 biases Ca 2+ signaling through lysosomes
    Article Snippet: For some experiments, TPC2-A1-N and TPC2-A1-P were purchased from MedChem Express.

    Triple Knockout:

    Article Title: Segregated cation flux by TPC2 biases Ca 2+ signaling through lysosomes
    Article Snippet: For some experiments, TPC2-A1-N and TPC2-A1-P were purchased from MedChem Express.

    Activity Assay:

    Article Title: Segregated cation flux by TPC2 biases Ca 2+ signaling through lysosomes
    Article Snippet: For some experiments, TPC2-A1-N and TPC2-A1-P were purchased from MedChem Express.

    CCK-8 Assay:

    Article Title: Segregated cation flux by TPC2 biases Ca 2+ signaling through lysosomes
    Article Snippet: For some experiments, TPC2-A1-N and TPC2-A1-P were purchased from MedChem Express.

    Confocal Microscopy:

    Article Title: Segregated cation flux by TPC2 biases Ca 2+ signaling through lysosomes
    Article Snippet: For some experiments, TPC2-A1-N and TPC2-A1-P were purchased from MedChem Express.

    Isolation:

    Article Title: Segregated cation flux by TPC2 biases Ca 2+ signaling through lysosomes
    Article Snippet: For some experiments, TPC2-A1-N and TPC2-A1-P were purchased from MedChem Express.

    Transfection:

    Article Title: Segregated cation flux by TPC2 biases Ca 2+ signaling through lysosomes
    Article Snippet: For some experiments, TPC2-A1-N and TPC2-A1-P were purchased from MedChem Express.

    Two Tailed Test:

    Article Title: Segregated cation flux by TPC2 biases Ca 2+ signaling through lysosomes
    Article Snippet: For some experiments, TPC2-A1-N and TPC2-A1-P were purchased from MedChem Express.

    Stable Transfection:

    Article Title: Segregated cation flux by TPC2 biases Ca 2+ signaling through lysosomes
    Article Snippet: For some experiments, TPC2-A1-N and TPC2-A1-P were purchased from MedChem Express.

    MANN-WHITNEY:

    Article Title: Segregated cation flux by TPC2 biases Ca 2+ signaling through lysosomes
    Article Snippet: For some experiments, TPC2-A1-N and TPC2-A1-P were purchased from MedChem Express.

    Mutagenesis:

    Article Title: Segregated cation flux by TPC2 biases Ca 2+ signaling through lysosomes
    Article Snippet: For some experiments, TPC2-A1-N and TPC2-A1-P were purchased from MedChem Express.

    Concentration Assay:

    Article Title: Segregated cation flux by TPC2 biases Ca 2+ signaling through lysosomes
    Article Snippet: For some experiments, TPC2-A1-N and TPC2-A1-P were purchased from MedChem Express.

    Derivative Assay:

    Article Title: Segregated cation flux by TPC2 biases Ca 2+ signaling through lysosomes
    Article Snippet: For some experiments, TPC2-A1-N and TPC2-A1-P were purchased from MedChem Express.

    CRISPR:

    Article Title: Segregated cation flux by TPC2 biases Ca 2+ signaling through lysosomes
    Article Snippet: For some experiments, TPC2-A1-N and TPC2-A1-P were purchased from MedChem Express.



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    A) WT larvae were incubated with specific agonists for 1h, then washed, and exposed to 50µM of a mixture of equal parts of G418-Bodipy and G418-pHrodo green for 1h before imaging. Representative images show G418-Bodipy (magenta) localizing to a large extent of vesicles in multiple hair cells, whereas high intensity G418-pHrodo green signal localizes in discrete regions of NM (Merge). Only TPC2-A1-N generates a lower pHrodo green fluorescence intensity. Dotted line delineates all hair cells in the NM. Scale bar: 10μm. B) Using G418-Bodipy fluorescent signal vesicles were segmented. Normalizing mean intensity by the volume of vesicles, integrated density was calculated on both channels, and mean vesicular ratiometric values were calculated. Each dot corresponds to a NM (average vesicle values), 3 NM per larva. 1h Pre-exposure to TPC2-A1-N neutralizes pH in G418-containing vesicles. Non-param. One-way ANOVA. C) Ratiometric values were calculates as previously described. 1h <t>Pre-exposure</t> <t>to</t> <t>TPC2-A1-P</t> doesn’t affect vesicular pH. Stats: Kruskal-Wallis One-way ANOVA, with Dunn’s multiple comparison test.
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    A) WT larvae were incubated with specific agonists for 1h, then washed, and exposed to 50µM of a mixture of equal parts of G418-Bodipy and G418-pHrodo green for 1h before imaging. Representative images show G418-Bodipy (magenta) localizing to a large extent of vesicles in multiple hair cells, whereas high intensity G418-pHrodo green signal localizes in discrete regions of NM (Merge). Only TPC2-A1-N generates a lower pHrodo green fluorescence intensity. Dotted line delineates all hair cells in the NM. Scale bar: 10μm. B) Using G418-Bodipy fluorescent signal vesicles were segmented. Normalizing mean intensity by the volume of vesicles, integrated density was calculated on both channels, and mean vesicular ratiometric values were calculated. Each dot corresponds to a NM (average vesicle values), 3 NM per larva. 1h Pre-exposure to TPC2-A1-N neutralizes pH in G418-containing vesicles. Non-param. One-way ANOVA. C) Ratiometric values were calculates as previously described. 1h <t>Pre-exposure</t> <t>to</t> <t>TPC2-A1-P</t> doesn’t affect vesicular pH. Stats: Kruskal-Wallis One-way ANOVA, with Dunn’s multiple comparison test.
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    Image Search Results


    A) WT larvae were incubated with specific agonists for 1h, then washed, and exposed to 50µM of a mixture of equal parts of G418-Bodipy and G418-pHrodo green for 1h before imaging. Representative images show G418-Bodipy (magenta) localizing to a large extent of vesicles in multiple hair cells, whereas high intensity G418-pHrodo green signal localizes in discrete regions of NM (Merge). Only TPC2-A1-N generates a lower pHrodo green fluorescence intensity. Dotted line delineates all hair cells in the NM. Scale bar: 10μm. B) Using G418-Bodipy fluorescent signal vesicles were segmented. Normalizing mean intensity by the volume of vesicles, integrated density was calculated on both channels, and mean vesicular ratiometric values were calculated. Each dot corresponds to a NM (average vesicle values), 3 NM per larva. 1h Pre-exposure to TPC2-A1-N neutralizes pH in G418-containing vesicles. Non-param. One-way ANOVA. C) Ratiometric values were calculates as previously described. 1h Pre-exposure to TPC2-A1-P doesn’t affect vesicular pH. Stats: Kruskal-Wallis One-way ANOVA, with Dunn’s multiple comparison test.

    Journal: bioRxiv

    Article Title: Lysosomal Ion Homeostasis Drives Delayed Hair Cell Death After Aminoglycoside Uptake

    doi: 10.64898/2026.06.10.731244

    Figure Lengend Snippet: A) WT larvae were incubated with specific agonists for 1h, then washed, and exposed to 50µM of a mixture of equal parts of G418-Bodipy and G418-pHrodo green for 1h before imaging. Representative images show G418-Bodipy (magenta) localizing to a large extent of vesicles in multiple hair cells, whereas high intensity G418-pHrodo green signal localizes in discrete regions of NM (Merge). Only TPC2-A1-N generates a lower pHrodo green fluorescence intensity. Dotted line delineates all hair cells in the NM. Scale bar: 10μm. B) Using G418-Bodipy fluorescent signal vesicles were segmented. Normalizing mean intensity by the volume of vesicles, integrated density was calculated on both channels, and mean vesicular ratiometric values were calculated. Each dot corresponds to a NM (average vesicle values), 3 NM per larva. 1h Pre-exposure to TPC2-A1-N neutralizes pH in G418-containing vesicles. Non-param. One-way ANOVA. C) Ratiometric values were calculates as previously described. 1h Pre-exposure to TPC2-A1-P doesn’t affect vesicular pH. Stats: Kruskal-Wallis One-way ANOVA, with Dunn’s multiple comparison test.

    Article Snippet: To test the TPC2 agonist TPC2-A1-N (Sigma-Aldrich, SML3562) and TPC2-A1-P (MedChemExpress, SML3700) compounds, larvae were preincubated for 1h in 3μM TPC2-A1-N and 10μM TPC2-A1-P, respectively.

    Techniques: Incubation, Imaging, Fluorescence, Comparison