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tpc2 a1 p  (MedChemExpress)


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    MedChemExpress tpc2 a1 p
    ( A, B ) Representative I–V relationships and corresponding summaries of <t>TPC2</t> PM currents activated by 40 µM (A) or 10 µM (B) <t>TPC2-A1-P.</t> Currents were recorded in the inside-out excised patch configuration in the absence or presence of 2 µM Lsm12 or 1 µM NAADP, as indicated. For summary plots, current amplitudes were measured at −120 mV and normalized to those recorded in the absence of Lsm12. Conditions are color-coded as indicated. ( C ) Representative I–V relationships of TPC2 PM currents activated by extracellularly applied TPC2-A1-P at the indicated concentrations and recorded in the cell-attached configuration from WT, Lsm12-KO, and Lsm12-reexpressing HEK293 cells. ( D ) Summary of TPC2 PM current amplitudes measured at −120 mV, shown as absolute amplitudes (left) or normalized to currents elicited by 40 µM TPC2-A1-P in the same recording (right). ( E ) Immunoblot of lysates from WT, Lsm12-KO, and Lsm12-reexpressing HEK293 cells probed with an anti-Lsm12 antibody. For Lsm12 reexpression, a C-terminal FLAG- and 6×His-tagged construct was used. Cell types are color-coded as indicated in (C–E). Bar graphs show individual data points and mean ± SEM. *, p < 0.05; **, p < 0.01; ***, p < 0.001; ns, p > 0.05.
    Tpc2 A1 P, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 94/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/tpc2+a1+p/bio_rxiv__64898__2026__04__13__718294-188-18-19?v=MedChemExpress
    Average 94 stars, based on 3 article reviews
    tpc2 a1 p - by Bioz Stars, 2026-08
    94/100 stars

    Images

    1) Product Images from "NAADP elicits two-pore channel currents by lifting Lsm12-mediated inhibition of PI(3,5)P 2 activation"

    Article Title: NAADP elicits two-pore channel currents by lifting Lsm12-mediated inhibition of PI(3,5)P 2 activation

    Journal: bioRxiv

    doi: 10.64898/2026.04.13.718294

    ( A, B ) Representative I–V relationships and corresponding summaries of TPC2 PM currents activated by 40 µM (A) or 10 µM (B) TPC2-A1-P. Currents were recorded in the inside-out excised patch configuration in the absence or presence of 2 µM Lsm12 or 1 µM NAADP, as indicated. For summary plots, current amplitudes were measured at −120 mV and normalized to those recorded in the absence of Lsm12. Conditions are color-coded as indicated. ( C ) Representative I–V relationships of TPC2 PM currents activated by extracellularly applied TPC2-A1-P at the indicated concentrations and recorded in the cell-attached configuration from WT, Lsm12-KO, and Lsm12-reexpressing HEK293 cells. ( D ) Summary of TPC2 PM current amplitudes measured at −120 mV, shown as absolute amplitudes (left) or normalized to currents elicited by 40 µM TPC2-A1-P in the same recording (right). ( E ) Immunoblot of lysates from WT, Lsm12-KO, and Lsm12-reexpressing HEK293 cells probed with an anti-Lsm12 antibody. For Lsm12 reexpression, a C-terminal FLAG- and 6×His-tagged construct was used. Cell types are color-coded as indicated in (C–E). Bar graphs show individual data points and mean ± SEM. *, p < 0.05; **, p < 0.01; ***, p < 0.001; ns, p > 0.05.
    Figure Legend Snippet: ( A, B ) Representative I–V relationships and corresponding summaries of TPC2 PM currents activated by 40 µM (A) or 10 µM (B) TPC2-A1-P. Currents were recorded in the inside-out excised patch configuration in the absence or presence of 2 µM Lsm12 or 1 µM NAADP, as indicated. For summary plots, current amplitudes were measured at −120 mV and normalized to those recorded in the absence of Lsm12. Conditions are color-coded as indicated. ( C ) Representative I–V relationships of TPC2 PM currents activated by extracellularly applied TPC2-A1-P at the indicated concentrations and recorded in the cell-attached configuration from WT, Lsm12-KO, and Lsm12-reexpressing HEK293 cells. ( D ) Summary of TPC2 PM current amplitudes measured at −120 mV, shown as absolute amplitudes (left) or normalized to currents elicited by 40 µM TPC2-A1-P in the same recording (right). ( E ) Immunoblot of lysates from WT, Lsm12-KO, and Lsm12-reexpressing HEK293 cells probed with an anti-Lsm12 antibody. For Lsm12 reexpression, a C-terminal FLAG- and 6×His-tagged construct was used. Cell types are color-coded as indicated in (C–E). Bar graphs show individual data points and mean ± SEM. *, p < 0.05; **, p < 0.01; ***, p < 0.001; ns, p > 0.05.

    Techniques Used: Western Blot, Construct

    Lsm12 knockout enhances sensitivity of TPC2-expressing cells to TPC2-A1-P–induced Ca 2+ elevation Left: Representative time courses of Ca 2+ indicator signals in response to 10 µM TPC2-A1-P in WT and Lsm12-KO HEK293 cells, with or without TPC2 expression. Right : Summary of peak Ca 2+ responses (ΔF/F₀) evoked by the indicated concentrations of TPC2-A1-P in WT and Lsm12-KO HEK293 cells, with or without TPC2 expression. Bar graphs show individual data points and mean ± SEM. **, p < 0.01; ***, p < 0.001; ****, p < 0.0001; ns, p > 0.05.
    Figure Legend Snippet: Lsm12 knockout enhances sensitivity of TPC2-expressing cells to TPC2-A1-P–induced Ca 2+ elevation Left: Representative time courses of Ca 2+ indicator signals in response to 10 µM TPC2-A1-P in WT and Lsm12-KO HEK293 cells, with or without TPC2 expression. Right : Summary of peak Ca 2+ responses (ΔF/F₀) evoked by the indicated concentrations of TPC2-A1-P in WT and Lsm12-KO HEK293 cells, with or without TPC2 expression. Bar graphs show individual data points and mean ± SEM. **, p < 0.01; ***, p < 0.001; ****, p < 0.0001; ns, p > 0.05.

    Techniques Used: Knock-Out, Expressing



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    ( A, B ) Representative I–V relationships and corresponding summaries of <t>TPC2</t> PM currents activated by 40 µM (A) or 10 µM (B) <t>TPC2-A1-P.</t> Currents were recorded in the inside-out excised patch configuration in the absence or presence of 2 µM Lsm12 or 1 µM NAADP, as indicated. For summary plots, current amplitudes were measured at −120 mV and normalized to those recorded in the absence of Lsm12. Conditions are color-coded as indicated. ( C ) Representative I–V relationships of TPC2 PM currents activated by extracellularly applied TPC2-A1-P at the indicated concentrations and recorded in the cell-attached configuration from WT, Lsm12-KO, and Lsm12-reexpressing HEK293 cells. ( D ) Summary of TPC2 PM current amplitudes measured at −120 mV, shown as absolute amplitudes (left) or normalized to currents elicited by 40 µM TPC2-A1-P in the same recording (right). ( E ) Immunoblot of lysates from WT, Lsm12-KO, and Lsm12-reexpressing HEK293 cells probed with an anti-Lsm12 antibody. For Lsm12 reexpression, a C-terminal FLAG- and 6×His-tagged construct was used. Cell types are color-coded as indicated in (C–E). Bar graphs show individual data points and mean ± SEM. *, p < 0.05; **, p < 0.01; ***, p < 0.001; ns, p > 0.05.
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    ( A, B ) Representative I–V relationships and corresponding summaries of <t>TPC2</t> PM currents activated by 40 µM (A) or 10 µM (B) <t>TPC2-A1-P.</t> Currents were recorded in the inside-out excised patch configuration in the absence or presence of 2 µM Lsm12 or 1 µM NAADP, as indicated. For summary plots, current amplitudes were measured at −120 mV and normalized to those recorded in the absence of Lsm12. Conditions are color-coded as indicated. ( C ) Representative I–V relationships of TPC2 PM currents activated by extracellularly applied TPC2-A1-P at the indicated concentrations and recorded in the cell-attached configuration from WT, Lsm12-KO, and Lsm12-reexpressing HEK293 cells. ( D ) Summary of TPC2 PM current amplitudes measured at −120 mV, shown as absolute amplitudes (left) or normalized to currents elicited by 40 µM TPC2-A1-P in the same recording (right). ( E ) Immunoblot of lysates from WT, Lsm12-KO, and Lsm12-reexpressing HEK293 cells probed with an anti-Lsm12 antibody. For Lsm12 reexpression, a C-terminal FLAG- and 6×His-tagged construct was used. Cell types are color-coded as indicated in (C–E). Bar graphs show individual data points and mean ± SEM. *, p < 0.05; **, p < 0.01; ***, p < 0.001; ns, p > 0.05.
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    ( A, B ) Representative I–V relationships and corresponding summaries of <t>TPC2</t> PM currents activated by 40 µM (A) or 10 µM (B) <t>TPC2-A1-P.</t> Currents were recorded in the inside-out excised patch configuration in the absence or presence of 2 µM Lsm12 or 1 µM NAADP, as indicated. For summary plots, current amplitudes were measured at −120 mV and normalized to those recorded in the absence of Lsm12. Conditions are color-coded as indicated. ( C ) Representative I–V relationships of TPC2 PM currents activated by extracellularly applied TPC2-A1-P at the indicated concentrations and recorded in the cell-attached configuration from WT, Lsm12-KO, and Lsm12-reexpressing HEK293 cells. ( D ) Summary of TPC2 PM current amplitudes measured at −120 mV, shown as absolute amplitudes (left) or normalized to currents elicited by 40 µM TPC2-A1-P in the same recording (right). ( E ) Immunoblot of lysates from WT, Lsm12-KO, and Lsm12-reexpressing HEK293 cells probed with an anti-Lsm12 antibody. For Lsm12 reexpression, a C-terminal FLAG- and 6×His-tagged construct was used. Cell types are color-coded as indicated in (C–E). Bar graphs show individual data points and mean ± SEM. *, p < 0.05; **, p < 0.01; ***, p < 0.001; ns, p > 0.05.
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    Image Search Results


    ( A, B ) Representative I–V relationships and corresponding summaries of TPC2 PM currents activated by 40 µM (A) or 10 µM (B) TPC2-A1-P. Currents were recorded in the inside-out excised patch configuration in the absence or presence of 2 µM Lsm12 or 1 µM NAADP, as indicated. For summary plots, current amplitudes were measured at −120 mV and normalized to those recorded in the absence of Lsm12. Conditions are color-coded as indicated. ( C ) Representative I–V relationships of TPC2 PM currents activated by extracellularly applied TPC2-A1-P at the indicated concentrations and recorded in the cell-attached configuration from WT, Lsm12-KO, and Lsm12-reexpressing HEK293 cells. ( D ) Summary of TPC2 PM current amplitudes measured at −120 mV, shown as absolute amplitudes (left) or normalized to currents elicited by 40 µM TPC2-A1-P in the same recording (right). ( E ) Immunoblot of lysates from WT, Lsm12-KO, and Lsm12-reexpressing HEK293 cells probed with an anti-Lsm12 antibody. For Lsm12 reexpression, a C-terminal FLAG- and 6×His-tagged construct was used. Cell types are color-coded as indicated in (C–E). Bar graphs show individual data points and mean ± SEM. *, p < 0.05; **, p < 0.01; ***, p < 0.001; ns, p > 0.05.

    Journal: bioRxiv

    Article Title: NAADP elicits two-pore channel currents by lifting Lsm12-mediated inhibition of PI(3,5)P 2 activation

    doi: 10.64898/2026.04.13.718294

    Figure Lengend Snippet: ( A, B ) Representative I–V relationships and corresponding summaries of TPC2 PM currents activated by 40 µM (A) or 10 µM (B) TPC2-A1-P. Currents were recorded in the inside-out excised patch configuration in the absence or presence of 2 µM Lsm12 or 1 µM NAADP, as indicated. For summary plots, current amplitudes were measured at −120 mV and normalized to those recorded in the absence of Lsm12. Conditions are color-coded as indicated. ( C ) Representative I–V relationships of TPC2 PM currents activated by extracellularly applied TPC2-A1-P at the indicated concentrations and recorded in the cell-attached configuration from WT, Lsm12-KO, and Lsm12-reexpressing HEK293 cells. ( D ) Summary of TPC2 PM current amplitudes measured at −120 mV, shown as absolute amplitudes (left) or normalized to currents elicited by 40 µM TPC2-A1-P in the same recording (right). ( E ) Immunoblot of lysates from WT, Lsm12-KO, and Lsm12-reexpressing HEK293 cells probed with an anti-Lsm12 antibody. For Lsm12 reexpression, a C-terminal FLAG- and 6×His-tagged construct was used. Cell types are color-coded as indicated in (C–E). Bar graphs show individual data points and mean ± SEM. *, p < 0.05; **, p < 0.01; ***, p < 0.001; ns, p > 0.05.

    Article Snippet: The TPC2 or TPC1 channel currents were elicited by perfusion of PtdIns( , )P2 diC8 (Echelon Biosciences #P-3058), TPC2-A1-P (MedChemExpress #HY-131615 or Sigma-Aldrich #SML3700), or TPC2-A1-N (MedChemExpress #HY-131614 or Sigma-Aldrich #SML3562) on the cytosolic side in inside-out recordings and whole endolysosomal recordings, or of TPC2-A1-P on the extracellular side in cell-attached recordings.

    Techniques: Western Blot, Construct

    Lsm12 knockout enhances sensitivity of TPC2-expressing cells to TPC2-A1-P–induced Ca 2+ elevation Left: Representative time courses of Ca 2+ indicator signals in response to 10 µM TPC2-A1-P in WT and Lsm12-KO HEK293 cells, with or without TPC2 expression. Right : Summary of peak Ca 2+ responses (ΔF/F₀) evoked by the indicated concentrations of TPC2-A1-P in WT and Lsm12-KO HEK293 cells, with or without TPC2 expression. Bar graphs show individual data points and mean ± SEM. **, p < 0.01; ***, p < 0.001; ****, p < 0.0001; ns, p > 0.05.

    Journal: bioRxiv

    Article Title: NAADP elicits two-pore channel currents by lifting Lsm12-mediated inhibition of PI(3,5)P 2 activation

    doi: 10.64898/2026.04.13.718294

    Figure Lengend Snippet: Lsm12 knockout enhances sensitivity of TPC2-expressing cells to TPC2-A1-P–induced Ca 2+ elevation Left: Representative time courses of Ca 2+ indicator signals in response to 10 µM TPC2-A1-P in WT and Lsm12-KO HEK293 cells, with or without TPC2 expression. Right : Summary of peak Ca 2+ responses (ΔF/F₀) evoked by the indicated concentrations of TPC2-A1-P in WT and Lsm12-KO HEK293 cells, with or without TPC2 expression. Bar graphs show individual data points and mean ± SEM. **, p < 0.01; ***, p < 0.001; ****, p < 0.0001; ns, p > 0.05.

    Article Snippet: The TPC2 or TPC1 channel currents were elicited by perfusion of PtdIns( , )P2 diC8 (Echelon Biosciences #P-3058), TPC2-A1-P (MedChemExpress #HY-131615 or Sigma-Aldrich #SML3700), or TPC2-A1-N (MedChemExpress #HY-131614 or Sigma-Aldrich #SML3562) on the cytosolic side in inside-out recordings and whole endolysosomal recordings, or of TPC2-A1-P on the extracellular side in cell-attached recordings.

    Techniques: Knock-Out, Expressing